2014•Methods in molecular biologyRequires access

In Vitro Selection of RNA Aptamers for a Small-Molecule Dye

Asako Murata, Shin‐ichi Sato

Abstract

Artificial riboswitches can be generated by functional coupling of an RNA aptamer for synthetic small molecule to an expression platform. RNA aptamers that can bind strongly and selectively to their target are feasible to be used for obtaining more potent artificial riboswitches. In this chapter, we describe tips and notes for in vitro selection of RNA aptamers targeting synthetic small molecules.

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Available abstract

Key concepts: Aptamer, Riboswitch, RNA, Computational biology, Small molecule, In vitro, SELEX Aptamer Technique, Systematic evolution of ligands by exponential enrichment