2009PubMedRequires access

[Analysis of SCCmec genotyping and antimicrobial susceptibility tests in methicillin resistant Staphylococcus aureus].

Sun Gui-zhen, Yan-Hua Yu, Xiuying Zhao

Open publisher page 0 citations

Abstract

OBJECTIVE: To investigate the SCCmec genotyping, subtype and antimicrobial susceptibility tests in methicillin resistant staphylococcus aureus to guide the clinical treatment and provide the proof for molecular epidemiology. METHODS: To detect mecA gene and SCCmec genetyping and subtype in 50 MRSA by PCR. According to CLSI's guideline, antimicrobial susceptibility tests were performed with disk diffusion. RESULTS: All 50 MRSA had mecA genes. 45 strains were SCCmec III types; 3 strains were SCCmec III A types; 2 strains were SCCmec II types. There were no SCCmec I and SCCmec IV types. SCCmec II, SCCmec III and SCCmec III A type strains were all multiresistant. CONCLUSION: 50 MRSA are all multiresistant. SCCmec III are the main types.

About this research paper

What this paper is about

OBJECTIVE: To investigate the SCCmec genotyping, subtype and antimicrobial susceptibility tests in methicillin resistant staphylococcus aureus to guide the clinical treatment and provide the proof for molecular epidemiology. METHODS: To detect mecA gene and SCCmec genetyping and subtype in 50 MRSA by PCR. According to CLSI's guideline, antimicrobial susceptibility tests were performed with disk diffusion. RESULTS: All 50 MRSA had mecA genes. 45 strains were SCCmec III types; 3 strains were SCCmec III A types; 2 strains were SCCmec II types. There were no SCCmec I and SCCmec IV types. SCCmec II, SCCmec III and SCCmec III A type strains were all multiresistant. CONCLUSION: 50 MRSA are all multiresistant. SCCmec III are the main types.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE: To investigate the SCCmec genotyping, subtype and antimicrobial susceptibility tests in methicillin resistant staphylococcus aureus to guide the clinical treatment and provide the proof for molecular epidemiology. METHODS: To detect mecA gene and SCCmec genetyping and subtype in 50 MRSA by PCR. According to CLSI's guideline, antimicrobial susceptibility tests were performed with disk diffusion. RESULTS: All 50 MRSA had mecA genes. 45 strains were SCCmec III types; 3 strains were SCCmec III A types; 2 strains were SCCmec II types. There were no SCCmec I and SCCmec IV types. SCCmec II, SCCmec III and SCCmec III A type strains were all multiresistant. CONCLUSION: 50 MRSA are all multiresistant. SCCmec III are the main types.

Key concepts: SCCmec, Staphylococcus aureus, Microbiology, Biology, Genotyping, Methicillin-resistant Staphylococcus aureus, Genotype, Gene

Related papers

Back to paper searchBrowse research topicsOriginal source
[Analysis of SCCmec genotyping and antimicrobial susceptibility tests in methicillin resistant Staphylococcus aureus]. — Research Paper | ScholarLens