2002•Analytical Science and TechnologyRequires access
Mutation Analysis in β 2 -Adrenergic Receptor Gene by Denaturing High Performance Liquid Chromatography (DHPLC)
Sang‐Bum Park, Chung‐Hun Oh, Jongwan Kim, Won‐Cheoul Jang
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Abstract
We analysed mutation of -adrenergic receptor gene that controls bronchial asthma by denaturing high performance liquid chromatography (DHPLC) according to ion-pair reversed-phase high performance liquid chromatography (IP-RP-HPLC). We extracted genomic DNA from 50 asthma patients, amplified DNA using PCR, and analysed PCR product by DHPLC. As a result, we obtained that mutation frequency was 15 (30%) among 50 cases. Consequently DHPLC mutation detection was confirmed that the result of direct sequencing was coincide exactly.